한국영양학회지(Korean J Nutr)2013;46(2):109 ~ 118
http://dx.doi.org/10.4163/kjn.2013.46.2.109
자외선 조사와 병행된 로얄제리 식이 공급이 무모 생쥐의 표피 보습과 필라그린, 유리아미노산 함량 및 관련 대사 효소의 발현 변화에 미치는 영향
민지현1·이윤주1·한상미2·조윤희1§
1경희대학교 동서의학대학원 의학영양학과,2농촌진흥청 농업생물부
Dietary effect of royal jelly supplementation on epidermal levels of hydration, filaggrins, free amino acids and the related enzyme expression in UV irradiated hairless mice*
Min, Jihyun1ㆍLee, Yunju1ㆍHan, Sang-Mi2ㆍChoi, Yunhi1§
1Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Korea
2Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon 441-100, Korea
ABSTRACT
Ultraviolet (UV)irradiation reduces epidermal hydration, which is paralleled by the reduction of natural moisturizing factors (NMFs). Of various NMFs, free amino acids (AAs)are major constituents generated by filaggrin degradation. In this study, we attempted to determine whether dietary supplementation of royal jelly (RJ)in UV-irradiated mice can alters epidermal levels of hydration, filaggrins, and free AAs as well as of peptidylarginine deiminase-3 (PAD3), an enzyme involved in filaggrin degradation processes. Albino hairless mice were fed either a control diet (group UV+:UV irradiated control)or diets with 1% RJ harvested from different areas in Korea (groups RJ1, RJ2, and RJ3)or imported from China (group RJ4)for six weeks in parallel with UV irradiation. A normal control group (group UV-)was fed a control diet without UV irradiation for six weeks. Reduced epidermal levels of hydration, total filaggrins, and PAD3 were observed in group UV+;in group RJ1, these levels were increased to a level similar to that of group UV-. In addition, profilaggrins, two repeat intermediates (2RI), a precursor with two filaggrin repeats, and filaggrin were increased. Although no alteration of AAs was observed in any of the groups, and glutamate and serine, major AAs of NMF in group RJ1 were higher than in group UV+. Despite the increased levels of PAD3, epidermal levels of hydration, filaggrins, glutamate, and
serine in groups RJ2, RJ3, and RJ4 were similar to those in group UV+. Dietary supplementation of RJ1 improves epidermal hydration in parallel with enhanced expression and degradation of filaggrin, but not by increased protein expression of PAD3, along with increased generation of glutamate and serine. (Korean J Nutr2013;46(2):109 ~ 118)
KEY WORDS: royal jelly, filaggrin, peptidylarginine deiminase-3 (PAD3), free amino acid, ultraviolet irradiation.
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민지현_한국영양학회_46(2)_109-118.pdf
